Transcapillary PO₂ gradients in contracting muscles across the fibre type and oxidative continuum

Abstract

In mixed fibre-type skeletal muscle transcapillary PO₂ gradients (PO₂ mv-PO₂ is; microvascular and interstitial, respectively) drive O₂ flux across the blood-myocyte interface where the greatest resistance to that O₂ flux resides. We assessed a broad spectrum of fibre-type and oxidative-capacity rat muscles across the rest-to-contraction (1 Hz, 120 s) transient to test the novel hypotheses that: (i) slow-twitch PO₂ is would be greater than fast-twitch, (ii) muscles with greater oxidative capacity have greater PO₂ is than glycolytic counterparts, and (iii) whether PO₂ mv-PO₂ is at rest is maintained during contractions across all muscle types. PO₂ mv and PO₂ is were determined via phosphorescence quenching in soleus (SOL; 91% type I+IIa fibres and CSa: ∼21 μmol min^-1 g^-1 ), peroneal (PER; 33% and ∼20 μmol min^-1 g^-1 ), mixed (MG; 9% and ∼26 μmol min^-1 g^-1 ) and white gastrocnemius (WG; 0% and ∼8 μmol min^-1 g^-1 ) across the rest-contraction transient. PO₂ mv was higher than PO₂ is in each muscle (∼6-13 mmHg; P < 0.05). SOL PO₂ is_area was greater than in the fast-twitch muscles during contractions (P < 0.05). Oxidative muscles had greater PO₂ is_nadir (9.4 ± 0.8, 7.4 ± 0.9 and 6.4 ± 0.4; SOL, PER and MG, respectively) than WG (3.0 ± 0.3 mmHg, P < 0.05). The magnitude of PO₂ mv-PO₂ is at rest decreased during contractions in MG only (∼11 to 7 mmHg; time × (PO₂ mv-PO₂ is) interaction, P < 0.05). These data support the hypothesis that, since transcapillary PO₂ gradients during contractions are maintained in all muscle types, increased O₂ flux must occur via enhanced intracapillary diffusing conductance, which is most extreme in highly oxidative fast-twitch muscle.