Halomonas lactosivorans sp. nov., isolated from salt-lake sediment

Abstract

A bacteria strain, designated CFH 90008^T, was isolated from a salt lake sediment sample collected from Yuncheng city, Shanxi Province, PR China. Strain CFH 90008^T was Gram-stain-negative, strictly aerobic, motile with lateral flagella and rod-shaped. Colonies were yellow, circular and smooth. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain CFH 90008^T belonged to the genus <i>Halomonas</i>, showing highest sequence similarity to <i>Halomonas daqingensis</i> DQD2-30^T (98.6 %), <i>Halomonas saliphila</i> LCB169^T (98.5 %), <i>Halomonas desiderata</i> FB2^T (98.1 %) and <i>Halomonas kenyensis</i> AIR-2^T (98.0 %). Good growth was observed at 10-50 °C, pH 6.0-9.0 and with NaCl concentration from 1.0 to 12.0 % (w/v). The predominant quinone was Q9. The major fatty acid (>10 %) was C_{18 : 1} ω7<i>c</i>, C_{16 : 0} and C_{16 : 1} ω7<i>c</i>. The genome of strain CFH 90008^T was 4.36 Mbp with a genomic DNA G+C content of 66.7 mol%. Based on low average nucleotide identity and DNA-DNAhybridization results, chemotaxonomic characteristics, and differential physiological properties, strain CFH 90008^T could not be classified into any recognized species of the genus <i>Halomonas</i>. Therefore, a new species, for which the name <i>Halomonas lactosivorans</i> sp. nov. is proposed. The type strain is CFH 90008^T (=DSM 103220^T=KCTC 52281^T).