Encapsulated Nanodroplet Crystallization of Organic-Soluble Small Molecules

Abstract

The routine crystallization of small molecules for single-crystal X-ray analysis remains a considerable experimental challenge. We report a general method for the high-throughput nanoscale crystallization of organic-soluble small molecules: encapsulated nanodroplet crystallization (ENaCt). ENaCt provides crystals suitable for X-ray analysis, allowing structural and de novo absolute stereochemical assignment for a diverse and challenging range of small molecules (bioactives, natural products, organometallics, etc.), as well as acting as a tool for new polymorph discovery.