Development and Validation of Stability Indicating HPLC Method for Determination of Caffeic Acid, Vitexin and Rosmarinic Acid in Thunbergia laurifolia Leaf Extract

Abstract

HPLC grade methanol (Fisher Scientific, UK), deionized water (DI) purified by Milli-Q water purification system (Adrona SIA, Latvia), sodium dihydrogen orthophosphate (Loba Chemie, India), phosphoric acid, hydrochloric acid and hydrogen peroxide (Fisher Scientific, UK) were used. CA, RA and vitexin (purify 98%) (Sigma, St. Louis,