An activity-based two-photon fluorescent probe for real-time and reversible imaging of oxidative stress in the rat brain

Abstract

The real-time and reversible detection of cellular glutathione and oxidative stress challenges the study of the redox homeostasis of biological systems. We report herein a modular approach to design the Michael addition between glutathione and coumarin derivatives for fluorescence imaging of the reversible and dynamic change of oxidative stress in living cells and the rat brain.