Chemotherapeutic Potential of L-Carnosine From Stimuli-Responsive Magnetic Nanoparticles Against Breast Cancer Model

Abstract

<b>Aim:</b> L-carnosine-coated magnetic nanoparticles (CCMNPs) were developed to enhance chemotherapeutic activity of carnosine-dipeptide. <b>Materials & methods:</b> Surface grafting of MNPs with carnosine was contended by differential scanning calorimetry, infrared spectroscopy and x-ray diffraction. Physicochemical characterization and <i>in vitro</i> cytotoxicity on MCF-7 cell line was carried out. <i>In vivo</i> chemotherapeutic activity and toxicity was assessed by an Ehrlich Ascites tumor model. <b>Results:</b> CCMNPs possessed monodispersed size (120 nm), ζ (-27.3 mV), magnetization (51.52 emu/g) and entrapment efficiency (88.3%) with sustained release rate. CCMNPs showed 2.3-folds lower IC₅₀ values compared with carnosine solution after 48 h. Targeted CCMNPs were specifically accumulated in tumor showing significant reduction in tumor size with no systemic toxicity. Significant reduction in VEGF and cyclin D1 levels were observed. <b>Conclusion:</b> The developed system endowed with responsiveness to an external stimulus can represent a promising magnetically targeted delivery system for carnosine site specific delivery.