Differentiation of Hybrid Progeny of Silver Carp (Hypophthalmichthys molitrix Val.) and Bighead Carp (H. nobilis Rich.) Based on Microsatellite Polymorphism

Abstract

The differentiation potential of 11 microsatellite loci Hmo11, Hmo13, Hmo15, Hmo25, Hmo26, Hmo31, Hmo33, Hmo34, Hmo36, Hmo37, Hmo40, to distinguish between silver carp ( Hypophthalmichthys molitrix Val.) and bighead carp ( H . nobilis Rich.) using molecular genetic analysis was assessed. The highest empirical F ST values were shown for the Hmo15 (0.5235), Hmo33 (0.4957), Hmo40 (0.3500), Hmo25 (0.3365), and Hmo31 (0.2458) STR loci. The accuracy of identification of bighead carp using these microsatellite loci was 99.5 ± 0.3% and of silver carp was 99.4 ± 0.8%. Using five STR loci, a scheme for hybrid identification between silver and bighead carps grown in aquaculture in the Republic of Belarus was proposed. This approach can be used as an express test in breeding and reproduction programs for these species of herbivorous fish.