Induced Pluripotent Stem Cell-Derived Smooth Muscle Cells Increase Angiogenesis and Accelerate Diabetic Wound Healing

Abstract

<b>Aim:</b> To assess the potential of human induced pluripotent stem cell-derived smooth muscle cells (hiPSC-SMC) to accelerate diabetic wound healing. <b>Methods:</b> hiPSC-SMC were embedded in 3D collagen scaffolds and cultured <i>in vitro</i> for 72 h; scaffolds were then applied to diabetic, nude mouse, splinted back wounds to assess <i>in vivo</i> healing. Cultured medium after scaffold incubation was collected and analyzed for expression of pro-angiogenic cytokines. <b>Results:</b> hiPSC-SMC secrete increased concentration of pro-angiogenic cytokines, compared with murine adipose derived stem cells. Delivery of hiPSC-SMC-containing collagen scaffolds accelerates diabetic wound healing and is associated with an increased number of total and M2 type macrophages. <b>Conclusion:</b> hiPSC-SMC promote angiogenesis and accelerate diabetic wound healing, making them a promising new candidate for treatment of diabetic wounds.