Cell Surface Energy Affects the Structure of Microalgal Biofilm

Abstract

Microalgae biofilm-based culture systems have wide applications in environmental engineering and biotechnology. Biofilm structure is critical for the transport of nutrients, gas, and signaling molecules in a microalgal biofilm. This work aims to understand the influence of cell surface energy (SE) on the microalgal biofilm structure. Three microalgae species were used as model cells in the study: <i>Chlorella</i> sp., <i>Nannochloris oculata</i>, and <i>Chlorella pyrenoidosa</i>. First, by mediating biofilm culture conditions, we obtained <i>Chlorella</i> sp. cells with SEs of 40.4 ± 1.5, 44.7 ± 1.0, and 62. 7 ± 1.2 mJ/m², <i>N. oculata</i> cells with SEs of 47.7 ± 0.5, 41.1 ± 1.0, and 62.6 ± 1.2 mJ/m², and <i>C. pyrenoidosa</i> cells with SEs of 64.0 ± 0.6, 62.1 ± 0.7, and 62.8 ± 0.6 mJ/m². Then, based on the characterizations of biofilm structures, we found that cell SE can significantly affect the microalgae biofilm structure. When the cell SEs ranged from 40 to 50 mJ/m², the microalgae cells formed heterogeneous biofilms with a large number of open voids, and the biofilm porosity was higher than 20%. Alternatively, when the cell SEs ranged from 50 to 65 mJ/m², the cells formed a flat, homogeneous biofilm with the porosity lower than 20%. Finally, the influencing mechanism of cell SE on biofilm structure was interpreted based on the thermodynamic theory via analyzing the co-adhesion energy between cells. The study has important implications in understanding factors that influence the biofilm structures.