Enzymatic-assisted polymerization of the lignin obtained from a macroalgae consortium, using an extracellular laccase-like enzyme (Tg-laccase) from<i>Tetraselmis gracilis</i>

Abstract

In the past decade, Mexican coasts have received an enormous influx of macroalgae species, producing serious environmental and public health concerns. Here, we developed a green methodology to generate a new polymer from the lignin contained in the macroalgae. The methodology consists in lignin extraction-by-boiling and its subsequent polymerization with a laccase-like enzyme from the green algae Tetraselmis gracilis (Tg-laccase). Mass spectrometry revealed the presence of guaiacyl (G), p-hydroxyphenyl (H), and sinapyl alcohol as the main monolignols in the lignin from Sargassum sp. On the other hand, MALDI-TOF spectra shows an increase in the size of the lignin chain after enzymatic polymerization process with Tg-laccase. Besides, the characterization of the novel polymer -using 1H NMR, FTIR, SEC-FPLC, and UV/Vis- allowed establishing that during the polymerization process there is a decrease in the number of phenolic groups as well as loss of aromatic protons, which allowed proposing a polimerizacion mechanism. This methodology could be promising in the development of a new lignin-based polymer and would open a new direction for the environmental management of the macroalgae on the Mexican beaches.