A genetically encoded GRAB sensor for measuring serotonin dynamics <i>in vivo</i>

Abstract

Abstract Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating a variety of processes in the brain. To directly visualize the dynamics of 5-HT, we developed a genetically encoded G PC R - A ctivation- B ased 5-HT (GRAB 5-HT ) sensor with high sensitivity, selectivity, and spatiotemporal resolution. GRAB 5-HT , detected 5-HT release in multiple physiological and pathological conditions in both flies and mice, and thus provides new insights into the dynamics and mechanisms of 5-HT signaling.