Nanozyme-Augmented Tumor Catalytic Therapy by Self-Supplied H₂O₂ Generation

Abstract

Nanozyme with the "dual identity" of enzymes and nanomaterials is an emerging strategy to mimic natural enzymes in bio-nanotechnology. However, how to improve the nanozyme-based therapeutic efficiency in vivo still remains a challenge. Herein, we fabricated a nanozyme platform (Fe@Fe₃O₄@Cu_2-<i>x</i>S, named as MNPs) possessing peroxidase enzyme-like activity and then introduced β-lapachone (La) to assemble an effective nanozyme (LaMNPs) to boost the production of reactive oxygen species and upregulate the level of H₂O₂ by the released La in an acidic tumor microenvironment. As a proof of concept, LaMNPs were injected intravenously into mice model, resulting in a significant and efficient inhibition of the tumor growth.