Exosomes derived from HeLa cells break down vascular integrity by triggering endoplasmic reticulum stress in endothelial cells

Abstract

ABSTRACT Exosomes play a critical role in intercellular communication since they contain signalling molecules and genetic materials. During tumorigenesis, tumour‐derived exosomes have been demonstrated to promote tumour angiogenesis and metastasis. However, how the exosomes facilitate tumour metastasis is not clear. Here we explored the effect of HeLa cell‐derived exosomes (Exo HeLa ) on endothelial tight junctions (TJ) and the related mechanisms. After human umbilical vein endothelial cells (HUVEC) were treated with Exo HeLa , TJ proteins zonula occludens‐1 (ZO‐1) and Claudin‐5 in HUVEC were significantly reduced as compared with that treated with exosomes from human cervical epithelial cells, while mRNA levels of ZO‐1 and Claudin‐5 remained unchanged. Consequently, permeability of endothelial monolayer was increased after the treatment with Exo HeLa . Injection of Exo HeLa into mice also increased vascular permeability and tumour metastasis in vivo . Neither knocking down of Dicer nor use of inhibitors of microRNAs targeting at mRNAs of ZO‐1 and Claudin‐5 could block the inhibitory effect of Exo HeLa on ZO‐1 and Claudin‐5. The expression of genes involved in endoplasmic reticulum (ER) stress was significantly increased in HUVECs after treated with Exo HeLa . Inhibition of ER stress by knocking down protein kinase RNA‐like endoplasmic reticulum kinase prevented the down‐regulation of ZO‐1 and Claudin‐5 by Exo HeLa . Our study found that HeLa cell‐derived exosomes promote metastasis by triggering ER stress in endothelial cells and break down endothelial integrity. Such effect of exosomes is microRNA‐independent.