Enhanced methane production by alleviating sulfide inhibition with a microbial electrolysis coupled anaerobic digestion reactor

Abstract

Anaerobic digestion (AD) of organics is a challenging task under high-strength sulfate (SO₄^2-) conditions. The generation of toxic sulfides by SO₄^2--reducing bacteria (SRB) causes low methane (CH₄) production. This study investigated the feasibility of alleviating sulfide inhibition and enhancing CH₄ production by using an anaerobic reactor with built-in microbial electrolysis cell (MEC), namely ME-AD reactor. Compared to AD reactor, unionized H₂S in the ME-AD reactor was sufficiently converted into ionized HS^- due to the weak alkaline condition created via cathodic H₂ production, which relieved the toxicity of unionized H₂S to methanogenesis. Correspondingly, the CH₄ production in the ME-AD system was 1.56 times higher than that in the AD reactor with alkaline-pH control and 3.03 times higher than that in the AD reactors (no external voltage and no electrodes) without alkaline-pH control. MEC increased the amount of substrates available for CH₄-producing bacteria (MPB) to generate more CH₄. Microbial community analysis indicated that hydrogentrophic MPB (e.g. Methanosphaera) and acetotrophic MPB (e.g. Methanosaeta) participated in the two major pathways of CH₄ formation were successfully enriched in the cathode biofilm and suspended sludge of the ME-AD system. Economic revenue from increased CH₄ production totally covered the cost of input electricity. Integration of MEC with AD could be an attractive technology to alleviate sulfide inhibition and enhance CH₄ production from AD of organics under SO₄^2--rich condition.