Mutual inhibition between Prkd2 and Bcl6 controls T follicular helper cell differentiation

Abstract

T follicular helper cells (T_FH) participate in germinal center (GC) development and are necessary for B cell production of high-affinity, isotype-switched antibodies. In a forward genetic screen, we identified a missense mutation in <i>Prkd2</i>, encoding the serine/threonine kinase protein kinase D2, which caused elevated titers of immunoglobulin E (IgE) in the serum. Subsequent analysis of serum antibodies in mice with a targeted null mutation of <i>Prkd2</i> demonstrated polyclonal hypergammaglobulinemia of IgE, IgG1, and IgA isotypes, which was exacerbated by the T cell-dependent humoral response to immunization. GC formation and GC B cells were increased in <i>Prkd2^-/-</i> spleens. These effects were the result of excessive cell-autonomous T_FH development caused by unrestricted Bcl6 nuclear translocation in <i>Prkd2^-/-</i> CD4⁺ T cells. Prkd2 directly binds to Bcl6, and Prkd2-dependent phosphorylation of Bcl6 is necessary to constrain Bcl6 to the cytoplasm, thereby limiting T_FH development. In response to immunization, Bcl6 repressed <i>Prkd2</i> expression in CD4⁺ T cells, thereby committing them to T_FH development. Thus, Prkd2 and Bcl6 form a mutually inhibitory positive feedback loop that controls the stable transition from naïve CD4⁺ T cells to T_FH during the adaptive immune response.