OpenSFDI: an open-source guide for constructing a spatial frequency domain imaging system

Abstract

<b>Significance</b>: Spatial frequency domain imaging (SFDI) is a diffuse optical measurement technique that can quantify tissue optical absorption (μ_a) and reduced scattering (<inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi>μ</mml:mi></mml:mrow><mml:mrow><mml:mi>s</mml:mi></mml:mrow><mml:mrow><mml:mo>'</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math></inline-formula>) on a pixel-by-pixel basis. Measurements of μ_a at different wavelengths enable the extraction of molar concentrations of tissue chromophores over a wide field, providing a noncontact and label-free means to assess tissue viability, oxygenation, microarchitecture, and molecular content. We present here openSFDI: an open-source guide for building a low-cost, small-footprint, three-wavelength SFDI system capable of quantifying μ_a and <inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi>μ</mml:mi></mml:mrow><mml:mrow><mml:mi>s</mml:mi></mml:mrow><mml:mrow><mml:mo>'</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math></inline-formula> as well as oxyhemoglobin and deoxyhemoglobin concentrations in biological tissue. The companion website provides a complete parts list along with detailed instructions for assembling the openSFDI system. <b>Aim</b>: We describe the design of openSFDI and report on the accuracy and precision of optical property extractions for three different systems fabricated according to the instructions on the openSFDI website. <b>Approach</b>: Accuracy was assessed by measuring nine tissue-simulating optical phantoms with a physiologically relevant range of μ_a and <inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi>μ</mml:mi></mml:mrow><mml:mrow><mml:mi>s</mml:mi></mml:mrow><mml:mrow><mml:mo>'</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math></inline-formula> with the openSFDI systems and a commercial SFDI device. Precision was assessed by repeatedly measuring the same phantom over 1 h. <b>Results</b>: The openSFDI systems had an error of 0 ± 6 % in μ_a and -2 ± 3 % in <inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi>μ</mml:mi></mml:mrow><mml:mrow><mml:mi>s</mml:mi></mml:mrow><mml:mrow><mml:mo>'</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math></inline-formula>, compared to a commercial SFDI system. Bland-Altman analysis revealed the limits of agreement between the two systems to be ± 0.004 mm^{- 1} for μ_a and -0.06 to 0.1 mm^{- 1} for <inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi>μ</mml:mi></mml:mrow><mml:mrow><mml:mi>s</mml:mi></mml:mrow><mml:mrow><mml:mo>'</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math></inline-formula>. The openSFDI system had low drift with an average standard deviation of 0.0007 mm^{- 1} and 0.05 mm^{- 1} in μ_a and <inline-formula><mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mrow><mml:mi>μ</mml:mi></mml:mrow><mml:mrow><mml:mi>s</mml:mi></mml:mrow><mml:mrow><mml:mo>'</mml:mo></mml:mrow></mml:msubsup></mml:mrow></mml:math></inline-formula>, respectively. , <b>Conclusion</b>: The openSFDI provides a customizable hardware platform for research groups seeking to utilize SFDI for quantitative diffuse optical imaging.