MiR-424 is over-expressed and attenuates ischemia-reperfusion kidney injury via p53 and death receptor 6 pathway.

Abstract

BACKGROUND: Ischemic reperfusion injury of kidney is major cause for renal failure, however the involved pathogenesis remains unclear creating an void for its effective treatment. Here we studied involvement of microRNA-424 in renal injury. METHODS: studies. Hoechst 33342 analysis was done for apoptosis. Blood urea nitrogen (BUN) and serum creatinine was done for renal function, Hematoxylin-eosin tissue damage and Terminal transferase-dUTP nick-end labeling assay for apoptosis. RT-PCR was done for miRNA and ChIP assay to identify the binding of p53 to miR-424. TargetScan and miRanda data base were scanned to find targets of miR-424. Protein expression was done by western blot analysis. RESULTS: . CONCLUSION: The study confirmed p53/miR-424/DR6 as a protective cascade during ischemic-reperfusion injury.