Pararobbsia silviterrae gen. nov., sp. nov., isolated from forest soil and reclassification of Burkholderia alpina as Pararobbsia alpina comb. nov.

Abstract

A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, DHC34 T , was isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, China (112° 31′ E 23° 10′ N). It grew optimally on R2A medium at 28 °C, at pH 6.0–7.0 and in the presence of 0–1 % (w/v) NaCl. Strain DHC34 T was closely related to Burkholderia alpina LMG 28138 T (98.5 % 16S rRNA gene sequence similarity). 16S rRNA gene sequence analysis showed that strain DHC34 T formed a clade with B. alpina LMG 28138 T , which is next to but branched deeply with Robbsia andropogonis ICMP 2807 T . The phylogenetic relationships among these three strains were also supported with the phylogram based on concatenated partial gyr B, rec A and trp B gene sequences. The phylogenomic tree generated with the UBCG tool showed that strains DHC34 T and R. andropogonis ICMP 2807 T were in a different clade. The DNA–DNA relatedness values between strain DHC34 T and B. alpina LMG 28138 T and R. andropogonis ICMP 2807 T were much lower than 70 %. Strain DHC34 T contained ubiquinone 8 as the major respiratory quinone. Its major fatty acids were C 16 : 0 , C 17 : 0 cyclo and C 19 : 0 cyclo ω8 c . The DNA G+C content of strain DHC34 T was 64.2 mol%. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, three unidentified aminophospholipids, four unidentified phospholipids, one unidentified aminolipid and a polar lipid. The phenotypic, phylogenetic, genotypic and chemotaxonomic data showed that strain DHC34 T represents a novel species of a new genus in the family Burkholderiaceae , for which the name Pararobbsia silviterrae gen. nov., sp. nov. is proposed. The type strain of Pararobbsia silviterrae is DHC34 T (=KCTC 42628 T =LMG 28845 T ). On the basis of the current data, Burkholderia alpina is renamed as Pararobbsia alpina comb. nov.