Proceedings of the National Academy of Sciences · 2019 · 33 citations · 33 references
Life SciencesMicrobiologyMolecular BiologyGene ExpressionMicrobial GeneticsMicrobial MetabolismBacterial Gene RegulationBacterial MetabolismBacterial PathogenesisGene Regulation In MicrobesGut MicrobiotaVitamin Biochemistry3' Untranslated Region RegulationCommensal MicrobiotaGenomic Stability MaintenanceGenomic StabilizationVitamin B12 (Cobalamin)Vitamin B12 BiosynthesisVitamin B12 HomeostasisVitamin B12 Metabolism
Significance Gut bacteria-associated metabolites pivotally maintain host immune and developmental homeostasis. Here we demonstrate that the cbiM Cbl riboswitch regulates expression of the cobA operon controlling bacterial VB12 biosynthesis. Molecular modification of this riboswitch significantly enhances VB12 production by the commensal Propionibacterium strain UF1. These findings outline the potential to further develop this bacterium, which has already demonstrated healthful benefits by repelling intestinal diseases, as an improved next-generation probiotic.
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