SEPARATION BY GEL FILTRATION AND MICRODETERMINATION OF UNBOUND BILIRUBIN

Abstract

Summary A gel filtration technique and a diazoreaction method for unbound bilirubin separation and determination, directly applicable to small blood volumes has been described. Precision of the microdetermination, reproducibility of the separation method and bilirubin recovery have been very satisfactory in our hands. The effects of pH and relative albumin concentration on the albumin‐bilirubin binding were studied in sera and in commercial albumin‐bilirubin mixtures.