Isolation and characterization of steroidal sapogenins and glycoalkaloids from tissue cultures of Solanum verbascifolium LINN.

Abstract

Undifferentiated callus tissue of S. verbascifolium LINN. was established from sterilized seeds on Murashige and Skoog's revised medium and maintained on the same medium. Six-month-old callus was then analyzed for steroidal sapogenins and glycoalkaloids. Diosgenin and solasodine were isolated and estimated quantitatively in tissue samples harvested periodically (2, 4, 6 and 8 weeks).