Kinematics motility of frozen-thawed X and Y sperm of Sumba Ongole bull

Abstract

Abstract The aim of this study was to investigate the kinematics motility of frozen-thawed Sumba sexed sperm of Ongole bull using snake head fish albumin (SFA) column method. The SFA was made by different concentration ratio of top and bottom fraction namely 2 and 4%; 3 and 5%; 4 and 6% respectively, and BSA 5 and 10% as control. Semen pellet was diluted with Optixcell CSS extender, then sexed sperms were filled in mini straw (0.25 mL) followed with equilibration at 4°C for 2-4 hours. Prefreezing was performed for 15 minutes then freeze in liquid nitrogen. Kinematics motility was evaluated using Computer Assisted Semen Analyzer (CASA: Spermvision TM 3.7.8) in parameter of total motility, progressive motility, distance average path (DAP), distance curvelinear (DCL), distance straight line (DSL), velocity average path (VAP), velocity curvelinear (VCL) and velocity straight line (VSL). The results of showed that sperms X (top fraction) of SFA treatment after frozen have better kinematics motility parameter compared to Y sperms, in total motility 42.13-64.09%, progressive motility 38.7-62.01%, VCL 55.89-61.85 µm/s, VAP 102.53-110.14 µm/s, and VSL 42.19-45.33 µm/s. In conclusion sperms resulted using SFA treatment (X sperm) have higher kinematic reflecting higher fertility capacity compared to control group.