PlWRKY13: A Transcription Factor Involved in Abiotic and Biotic Stress Responses in Paeonia lactiflora

Abstract

Many members of the WRKY family regulate plant growth and development. Recent studies have shown that members of the WRKY family, specifically WRKY13, play various roles in the regulation of plant stress resistance. To study the function of WRKY family members in peony, the <i>PlWRKY13</i> gene (KY271095) was cloned from peony leaves. Sequence analysis and subcellular localization results revealed that <i>PlWRKY13</i> has no introns, belongs to the type IIc subgroup of the WRKY family, and functions in the nucleus. The expression pattern of <i>PlWRKY13</i> was analysed via real-time quantitative RT-PCR (qRT-PCR), which showed that the expression of <i>PlWRKY13</i> was induced by four types of abiotic stress, low-temperature, high-temperature, waterlogging and salt stress, and was positively upregulated in response to these stresses. In addition, the expression of <i>PlWRKY13</i> tended to first decrease and then increase after infection with <i>Alternaria tenuissima</i>. Virus-induced gene silencing (VIGS) technology was used to explore the function of <i>PlWRKY13</i> in the resistance of <i>Paeonia lactiflora</i> to fungal infection further, and the results showed that <i>PlWRKY13</i>-silenced plants displayed increased sensitivity to <i>A. tenuissima.</i> The infection was more severe and the disease index (DI) significantly greater in the <i>PlWRKY13</i>-silenced plants than in the control plants, and the expression of pathogenesis-related (PR) genes was also significantly altered in the <i>PlWRKY13</i>-silenced plants compared with the control plants. The contents of the endogenous hormones jasmonic acid (JA) and salicylic acid (SA) were measured, and the results showed that the JA content increased gradually after infection with <i>A. tenuissima</i> and that JA may play an active role in the resistance of <i>P. lactiflora</i> to pathogen infection, while the SA content decreased after <i>PlWRKY13</i> silencing. The contents of the two hormones decreased overall, suggesting that they are related to the transcription of <i>PlWRKY13</i> and that <i>PlWRKY13</i> may be involved in the disease-resistance pathway mediated by JA and SA. In summary, the results of our study showed that <i>PlWRKY13</i> expression was induced by stress and had a positive effect on the resistance of <i>P. lactiflora</i> to fungal infection.