Comparative genomic analysis of the <i>PAL</i> genes in five Rosaceae species and functional identification of Chinese white pear

Abstract

Phenylalanine ammonia lyase (PAL) plays an important role in the biosynthesis of secondary metabolites regulating plant growth response. To date, the evolutionary history of the PAL family in Rosaceae plants remains unclear. In this study, we identified 16 PAL homologous genes in five Rosaceae plants ( Pyrus bretschneideri , Fragaria vesca , Prunus mume , Prunus persica , and Malus × domestica ). We classified these PAL s into three categories based on phylogenetic analysis, and all PAL s were distributed on 13 chromosomes. We tracked gene duplication events and performed sliding window analysis. These results revealed the evolution of PAL s in five Rosaceae plants. We predicted the promoter of the PbPAL s by PLANT CARE online software, and found that the promoter region of both PbPAL1 and PbPAL3 have at least one AC element. The results of qRT-PCR analysis found that PbPAL1 and PbPAL2 were highly expressed in the stems and roots, while expression level of PbPAL3 was relatively low in different tissues. The expression of PbPAL1 and PbPAL2 increased firstly and then decreased at different developmental periods of pear fruit. Among them, the expression of PbPAL1 reached the highest level 55 days after flowering. Three PbPAL s were induced by abiotic stress to varying degrees. We transfected PbPAL1 and PbPAL2 into Arabidopsis thaliana , which resulted in an increase in lignin content and thickening of the cell walls of intervascular fibres and xylem cells. In summary, this research laid a foundation for better understanding the molecular evolution of PAL s in five Rosaceae plants. Furthermore, the present study revealed the role of PbPAL s in lignin synthesis, and provided basic data for regulating lignin synthesis and stone cells development in pear plants.