Chemical Communications · 2019 · 25 citations · 25 references
EngineeringBiochemistryMolecular BiologyBiosensorsNanobiotechnologyNanotechnologyEnzymatic BiosensorsEnzyme AssaysEnzyme-based BiosensorsEnzyme-linked Immunosorbent AssayNanomaterial-based BiosensorsNanosensorsEnzyme NanobiosensorsEnzyme-based NanobiosensorsEnzymesGold Nanoparticle BiosensorsGold Nanoparticle DetectionMethod Detection LimitNanoparticle-based BiosensorsProtein Tyrosine Phosphatase Inhibitors
We developed a peptide-templated gold nanoparticle (AuNP) nanosensor for simultaneous detection of multiple posttranslational modification (PTM) enzymes with a detection limit of 28 pM for histone deacetylase (HDAC) and 0.8 pM for protein tyrosine phosphatase 1B (PTP1B), and it can be further applied for the screening of PTM enzyme inhibitors and the measurement of PTM enzymes in cancer cells.
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Liang Hong, Gary P. Schroth, H.R. Matthews et al. · Journal of Biological Chemistry · 1993 · 424 citations · Full text