Maturation of the Goat Rumen Microbiota Involves Three Stages of Microbial Colonization

Abstract

With increasing age, the rumen microbiota of new-born ruminants become central in the translation of fibrous feed substances into essential nutrients. However, the colonization process of the microbial community (especially fungal community) remains poorly understood in ruminants at pre-weaning stages. In this study, the rumen bacterial and fungal colonization processes were investigated in goats at eight stages using amplicon sequencing. For bacteria, we found 36 common core genera at D0, D3, D14, D28, and D56, including mainly <i>Bacillus</i>, <i>Alloprevotella</i>, <i>Bacteroides</i>, <i>Prevotella_1</i>, <i>Lactococcus</i><i>,</i> and <i>Ruminococcaceae_NK4A214</i>. Firmicutes was the dominant phylum among the total microbiota in newborn goat kids (prior to nursing), while <i>Bacillus</i>, <i>Lactococcus</i>, and <i>Pseudomonas</i> were predominant genera. Interestingly, the proportion of <i>Bacillus</i> was as high as 55% in newborn animals. After milk nursing, the predominant phylum changed to Bacteroidetes, while the proportion of <i>Bacillus</i> and <i>Lactobacillus</i> was very low. CowPi was used to predict the functional gene pathways and we found increases in the abundance of genes associated with amino acid related enzymes, DNA repair and recombination proteins, aminoacyl tRNA biosynthesis, and peptidases after D3. With regard to fungi, we found that there were 51 common genera at day 0 (D0), D3, D14, D28, and D56, including mainly <i>Cryptococcus</i>, <i>Aspergillus</i>, and <i>Caecomyces</i>. <i>Aspergillus</i> occupied approximately 47% at day 0, but then it decreased from day 3 to day 14. This study indicates that the core microbes of rumen emerged shortly after birth, but the abundance was very different from the core genus of the adult rumen. In addition, we also report a detailed scheme of the bacterial and fungal colonization process in rumens and propose three distinct stages during the rumen colonization process in pre-weaning goats, which will offer a reference for the development of milk substitutes for small ruminants.