METABOLIC EPIDEMIOLOGY OF COLON CANCER - EFFECT OF DIETARY FIBER ON FECAL MUTAGENS AND BILE-ACIDS IN HEALTHY-SUBJECTS

Abstract

Because of potential significance of fecal mutagens and secondary bile acids in the pathogenesis of colonie cancer and of inverse association between dietary fiber and colonie cancer risk, the effect of dietary wheat and rye fiber on fecal mutagenic activity and bile acid levels was studied in 15 healthy men and women who were consuming high fat/moderately low fiber diets and excreting high levels of fecal mutagens and bile acids. Each participant provided two 24-h stool specimens and a 3-day diet record while consuming their normal diet (control). All subjects were then asked to consume their normal diet plus 11 g of supplemental fiber per day in the form of whole grain bread for 4 weeks. During the last week of diet intervention, each subject provided two 24-h stool specimens and a 3-day dietary record. Fecal samples collected from both periods were analyzed for bile acids and for mutagens using Salmonella typhimurium strains TA98 and TA 100 with and without nticrosomal activa tion. The concentration of fecal secondary bile acids was significantly lower during the fiber supplemental period in all subjects. Fiber supple mentation also inhibited the fecal mutagenic activity in IA100 and TA98 with and without microsomal activation. Thus, the increased fiber intake in the form of whole wheat and rye bread may reduce the production and/ or excretion of fecal mutagens and decrease the concentration of fecal secondary bile acids in humans.