MicroRNA‑1296 expression is associated with prognosis and inhibits cell proliferation and invasion by Wnt signaling in non‑small cell lung cancer

Abstract

MicroRNAs (miRNAs) are vital regulators of non‑small cell lung cancer (NSCLC) development and tumorigenesis. The aim of the present study was to explore the role of miRNA (miR)‑1296 expression in NSCLC. The expression of miR‑1296 was detected by reverse transcription‑quantitative PCR in NSCLC tissues and matched normal tissues. The association of miR‑1296 expression with clinicopathological factors of NSCLC patients was evaluated by the χ2 test. Prognostic value of miR‑1296 expression levels in patients with NSCLC was assessed using the Kaplan‑Meier method and a Cox proportional hazards model; Cell Counting Kit‑8, Transwell migration and western blot assays were used to detect the association between miR‑1296 and cell proliferation, invasion and Wnt signaling in NSCLC, respectively. The results of the present study demonstrated that miR‑1296 expression was significantly downregulated in NSCLC tissues and cells compared to corresponding controls. Lower miR‑1296 expression exhibited a significant association with lymph node metastasis and tumor‑node‑metastasis stage of patients with NSCLC. In addition, the survival analysis demonstrated that low miR‑1296 expression predicted a poorer prognosis compared to high miR‑1296 expression. Multivariate Cox analysis also demonstrated that reduced miR‑1296 expression was an independent risk factor of NSCLC prognosis. Additionally, miR‑1296 inhibited cell proliferation, invasion and Wnt signaling in NSCLC. Thus, the results of the present study indicated that miR‑1296 expression may be a potential biomarker of NSCLC prognosis and potential target for NSCLC treatment.