Streptomyces paludis sp. nov., isolated from an alpine wetland soil

Abstract

A Gram-stain-positive actinobacterium, designated strain GSSD-12^T, was isolated from an alpine wetland soil. blast search of the full-length 16S rRNA gene sequence of GSSD-12^T indicated it represented a member of the genus <i>Streptomyces</i>, and displayed highest similarity with <i>Streptomyces scopuliridis</i> NRRL B-24574^T (99.2 %) and less than 98.6 % similarity with other species of the genus <i>Streptomyces</i> with validly published names. Phylogenetic analysis based on 16S rRNA gene sequences indicated that GSSD-12^T was closely related to <i>S. scopuliridis</i> NRRL B-24574^T, <i>Streptomyces odonnellii</i> NRRL B-24891^T and <i>Streptomyces lushanensis</i> NRRL B-24994^T. However, the digital DNA-DNA hybridization value, the average nucleotide identity value and the multilocus sequence analysis evolutionary distance between this strain and its closest relatives indicated that it represented a distinct species. Furthermore, GSSD-12^T was also distinctly differentiated from them by morphological, physiological and biochemical characteristics. Therefore, strain GSSD-12^T(=CICC 11051^T=JCM 33019^T) represents a novel species of the genus <i>Streptomyces</i>, for which the name <i>Streptomyces paludis</i> sp. nov. is proposed.