Absicoccus porci gen. nov., sp. nov., a member of the family Erysipelotrichaceae isolated from pig faeces

Abstract

An obligately anaerobic, Gram-stain-positive and coccus-shaped bacterium, designated strain YH-panp20^T, was isolated from pig faeces. Phylogenetic analysis based on 16S rRNA gene sequencing indicated that the isolate belongs to the family <i>Erysipelotrichaceae</i>, and is most closely related to <i>Catenisphaera adipataccumulans</i> KCTC 15517^T (93.5 % sequence similarity), followed by <i>Faecalitalea cylindroides</i> KCTC 5815^T (92.2 %), <i>Faecalicoccus acidiformans</i> KCTC 15521^T (90.2 %) and <i>Holdemanella biformis</i> KCTC 5969^T (89.6 %). Average nucleotide identity values between YH-panp20^T and its closest relatives were lower than 71 %. The G+C content of the isolate was 38.4 mol%, and its cell-wall peptidoglycan was found to be of A1γ type and contained <i>meso</i>-diaminopimelic acid. The predominant fatty acids were C_{18 : 1} <i>cis</i> 9, C_{18 : 0} DMA and C_{16 : 0}. The major end-products of glucose fermentation were lactate, acetate and formate. Therefore, based on the phenotypic, phylogenetic and chemotaxonomic properties, a novel genus and species, <i>Absicoccus porci</i> gen. nov., sp. nov., is proposed for isolate YH-panp20^T (=KCTC 15747^T=JCM 32769^T).