Influence of PGPB Inoculation on HSP70 and HMA3 Gene Expression in Switchgrass under Cadmium Stress

Abstract

This study aimed to evaluate the gene expression of <i>HSP70</i> and <i>HMA3</i> in the switchgrass inoculated with plant-growth-promoting-bacteria (PGPB) under cadmium (Cd) stress and to observe the benefit of PGPB in plant growth and development. Plants were grown in hydroponic culture and treated with PGPB inoculants: <i>Pseudomonas grimontii, Pantoea vagans, Pseudomonas veronii,</i> and <i>Pseudomonas fluorescens</i> with the strains Bc09, So23, E02, and Oj24, respectively. The experimental results revealed that <i>HSP70</i> and <i>HMA3</i> genes expressed highly in the PGPB-inoculated plants under Cd stress. In addition, the expression of <i>HSP70</i> and <i>HMA3</i> genes was considerably higher in the first two days after successive four-day exposure of Cd in plants compared to the last two days of exposure. Increased biomass and indole-3-acetic-acid production with reduced Cd accumulation were observed in the PGPB-inoculated plants under Cd stress compared to the Cd-control plants. These PGPB, with their beneficial mechanisms, protect plants by modifying the gene expression profile that arises during Cd-toxic conditions and increased the healthy biomass of switchgrass. This demonstrates there is a correlation among the growth parameters under Cd stress. The PGPB in this study may help to intensify agriculture by triggering mechanisms to encourage plant growth and development under heavy metal stress.