Characteristics of Gut Microbiota in Patients With Rheumatoid Arthritis in Shanghai, China

Abstract

Little is known regarding differences in the gut microbiomes of rheumatoid arthritis (RA) patients and healthy cohorts in China. This study aimed to identify differences in the fecal microbiomes of 66 Chinese patients with RA and 60 healthy Chinese controls. The V3-V4 variable regions of bacterial 16S rRNA genes were sequenced with the Illumina system to define the bacterial composition. The alpha-diversity index of the microbiome of the RA patients was significantly lower than that of the control group. The bacterial genera <i>Bacteroides</i> (<i>p</i> = 0.02202) and <i>Escherichia-Shigella</i> (<i>p</i> = 0.03137) were more abundant in RA patients. In contrast, <i>Lactobacillus</i> (<i>p</i> = 0.000014)<i>, Alloprevotella</i> (<i>p</i> = 0.0000008615)<i>, Enterobacter</i> (<i>p</i> = 0.000005759), and <i>Odoribacter</i> (<i>p</i> = 0.0000166) were less abundant in the RA group than in the control group. Spearman correlation analysis of blood physiological measures of RA showed that bacterial genera such as <i>Dorea</i> and <i>Ruminococcus</i> were positively correlated with RF-IgA and anti-CCP antibodies. Furthermore, <i>Alloprevotella</i> and <i>Parabacteroides</i> were positively correlated with the erythrocyte sedimentation rate, and <i>Prevotella</i>-2 and <i>Alloprevotella</i> were positively correlated with C-reactive protein, both biomarkers of inflammation. These findings suggest that the gut microbiota may contribute to RA development via interactions with the host immune system.