PLANT PHYSIOLOGY · 2019 · 307 citations · 49 references
Life SciencesPlant BiologyPlant SciencesGene ExpressionPhotosynthesisPlant PhysiologyAbiotic Stress ToleranceHeat Stress TolerancePhotosynthetic Gene ExpressionPhotosystem IPlant Hormone SignalingPlant Transcriptional RegulationHigh-light StressLight StressPhotosynthetic Stress ResponsesProtein Expression LevelsStress Response Transcriptional StatesStress-induced Photosynthetic Gene ExpressionStress-induced ToleranceTranscripts
) plants to a combination of HL and HS (HL+HS) conditions. Combined HL+HS was accompanied by irreversible damage to PSII, decreased D1 (PsbA) protein levels, and an enhanced transcriptional response indicative of PSII repair activation. We further identified several unique aspects of this stress combination that included enhanced accumulation of jasmonic acid (JA) and JA-Ile, elevated expression of over 2,200 different transcripts that are unique to the stress combination (including many that are JA-associated), and distinctive structural changes to chloroplasts. A mutant deficient in JA biosynthesis (allene oxide synthase) displayed enhanced sensitivity to combined HL+HS and further analysis revealed that JA is required for regulating several transcriptional responses unique to the stress combination. Our study reveals that JA plays an important role in the acclimation of plants to a combination of HL+HS.
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STAR: ultrafast universal RNA-seq aligner
Alexander Dobin, Carrie Davis, Felix Schlesinger et al. · Bioinformatics · 2012 · 56.2K citations · Full text