Complete mitochondrial genome sequence of <i>Labriocimbex sinicus</i> , a new genus and new species of Cimbicidae (Hymenoptera) from China

Abstract

<i>Labriocimbex sinicus</i> Yan & Wei gen. et sp. nov. of Cimbicidae is described. The new genus is similar to <i>Praia</i> Andre and <i>Trichiosoma</i> Leach. A key to extant Holarctic genera of Cimbicinae is provided. To identify the phylogenetic placement of Cimbicidae, the mitochondrial genome of <i>L. sinicus</i> was annotated and characterized using high-throughput sequencing data. The complete mitochondrial genome of <i>L. sinicus</i> was obtained with a length of 15,405 bp (GenBank: MH136623; SRA: SRR8270383) and a typical set of 37 genes (22 tRNAs, 13 PCGs, and two rRNAs). The results demonstrated that all PCGs were initiated by ATN codon, and ended with TAA or T stop codons. The study reveals that all tRNA genes have a typical clover-leaf secondary structure, except for <i>trnS1</i>. Remarkably, the secondary structures of the <i>rrnS</i> and <i>rrnL</i> of <i>L. sinicus</i> were much different from those of <i>Corynis lateralis</i>. Phylogenetic analyses verified the monophyly and positions of the three Cimbicidae species within the superfamily Tenthredinoidea and demonstrated a relationship as (Tenthredinidae + Cimbicidae) + (Argidae + Pergidae) with strong nodal supports. Furthermore, we found that the generic relationships of Cimbicidae revealed by the phylogenetic analyses based on <i>COI</i> genes agree quite closely with the systematic arrangement of the genera based on the morphological characters. Phylogenetic tree based on two methods shows that <i>L. sinicus</i> is the sister group of <i>Praia</i> with high support values. We suggest that <i>Labriocimbex</i> belongs to the tribe Trichiosomini of Cimbicinae based on adult morphology and molecular data. Besides, we suggest to promote the subgenus <i>Asitrichiosoma</i> to be a valid genus.