An Intact ACTH LC-MS/MS Assay as an Arbiter of Clinically Discordant Immunoassay Results

Abstract

Immunoprecipitation of biologically active ACTH molecules followed by LC-MS/MS analysis enabled selective detection of iACTH and relevant biologically active fragments in plasma. Applied to the investigation of clinically discrepant results, this method can act as an arbiter of the concentration of iACTH present.