Effective editing for lysophosphatidic acid acyltransferase 2/5 in allotetraploid rapeseed (Brassica napus L.) using CRISPR-Cas9 system

Abstract

Seven <i>BnLPAT2</i> homologous genes and four <i>BnLPAT5</i> homologous genes were cleaved completely using the CRISPR-Cas9 system, which indicated that it is effective for editing all homologous genes in allotetraploid rapeseed, despite the relatively low sequence identities of both gene families. The size of the oil bodies increased significantly while the oil content decreased, confirming that <i>BnLPAT2</i> and <i>BnLPAT5</i> play a role in oil biosynthesis. The present study lays a foundation for further oil production improvement in oilseed crop species.