First Report of <i>bla</i> _VIM-4 - and <i>mcr-9</i> -Coharboring <i>Enterobacter</i> Species Isolated from a Pediatric Patient

Abstract

An <i>Enterobacter hormaechei</i> isolate harboring <i>bla</i>_VIM-4 and <i>mcr-9</i> was recovered from a pediatric patient in a U.S. hospital. The <i>bla</i>_VIM-4 and <i>mcr-9</i> genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The <i>mcr-9</i> gene is located between two insertion elements, IS<i>903</i> and IS<i>1</i>, but lacks the downstream regulatory genes (<i>qseC</i> and <i>qseB</i>) found in other isolates that harbor <i>mcr-9</i><b>IMPORTANCE</b> We describe the complete genome assembly and sequence of a clinical <i>Enterobacter</i> isolate harboring both <i>bla</i>_VIM-4 and <i>mcr-9</i> recovered from a pediatric patient in the United States with a history of travel to Egypt. Moreover, to the best of our knowledge, this is the first report of an <i>Enterobacter</i> isolate harboring both <i>bla</i>_VIM-4 and <i>mcr-9</i> from the United States. The <i>bla</i>_VIM-4 and <i>mcr-9</i> genes are carried on the same IncH12 plasmid, pME-1a. The isolate tested susceptible to colistin, without observed induction of colistin resistance. The <i>mcr-9</i> gene is located between two insertion elements, IS<i>903</i> and IS<i>1</i>, but lacks the downstream regulatory genes (<i>qseC</i> and <i>qseB</i>) found in other isolates that harbor <i>mcr-9</i>.