Rhodoferax bucti sp. nov., isolated from fresh water

Abstract

A Gram-reaction-negative, peach-brown-pigmented, slightly curved-rod-shaped, aerobic, non-motile bacterium, designated GSA243-2^T, was isolated from fresh water samples collected from the Chishui River flowing through Maotai, Guizhou, south-west PR China. Phenotypic, chemotaxonomic and genomic traits were investigated. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belonged to the genus <i>Rhodoferax</i>. The closest phylogenetic relative was <i>Rhodoferax saidenbachensis</i> ATCC BAA-1852^T (98.35 %). The major fatty acids were C_{16: 0} and C_{16 : 1}ω6<i>c</i> and/or C_{16 : 1}ω7<i>c</i>. The major respiratory quinone was ubiquinone Q-8 and the major polar lipid was phosphatidylethanolamine. Genome sequencing revealed a genome size of 3.67 Mbp and a G+C content of 61.17 mol%. Pairwise-determined whole genome average nucleotide identity values and digital DNA-DNA hybridization values suggested that strain GSA243-2^T represents a new species, for which we propose the name <i>Rhodoferaxbucti</i> sp. nov. with the type strain GSA243-2^T (=CGMCC 1.16288^T=KCTC 62564^T).