Biosurfactants of <i>Lactobacillus Helveticus</i> for Biodiversity Inhibit the Biofilm Formation of <i>Staphylococcus Aureus</i> and Cell Invasion

Abstract

<b>Aim:</b> This study aimed to evaluate the differences of biosurfactants produced by two <i>Lactobacillus helveticus</i> strains against the biofilm formation of <i>Staphylococcus aureus in vitro</i> and <i>in vivo</i>. <b>Materials & methods:</b> Scanning electron microscopy, Real time-quantitative PCR (RT-qPCR) and cell assay were used to analyze the inhibiting effect of biosurfactants against biofilm formation. <b>Results & conclusion:</b> Results showed that the biosurfactants have anti-adhesive and inhibiting effects on biofilm formation <i>in vivo</i> and <i>in vitro</i>. The biofilm-formative genes and autoinducer-2 signaling regulated these characteristics, and the biosurfactant <i>L. helveticus</i> 27170 is better than that of 27058. Host cell adhesion and invasion results indicated that the biosurfactants <i>L. helveticus</i> prevented the <i>S. aureus</i> invading the host cell, which may be a new strategy to eliminate biofilms.