The effect of ozone on hypoxia, hemolysis and morphological change of blood from patients with aortic dissection (AD): a preliminary <i>in vitro</i> experiment of ozonated autohemotherapy for treating AD.

Abstract

increased under Ozone concentrations at 40 μg/mL, 80 μg/mL and 160 μg/mL compared with samples exposed to 0 μg/mL in both AD group and control group. The MDA level was similar in samples exposed to 0 μg/mL, 40 μg/mL and 80 μg/mL ozone, while the levels of SOD increased in samples exposed to 40 μg/mL and 80 μg/mL in both AD group and control group. Compared with the samples exposed to 0 μg/mL ozone, FHb level only increased in samples exposed to 80 μg/mL and 160 μg/mL Ozone in both AD group and control group. In addition, overdosed ozone (160 μg/mL) but not therapeutic ozone concentrations (0 μg/mL, 40 μg/mL and 80 μg/mL) increased malformation percentage and morphology change of erythrocytes in both AD group and control group. In conclusion, Ozone improves oxygen content and reduces oxidative damage in blood from AD patients, and therapeutic dose ozone do not induce hemolysis and morphology change of erythrocytes.