Functional analysis of isoprenoid precursors biosynthesis by quantitative metabolomics and isotopologue profiling

Abstract

This methodology fills one of the last technical gaps for functional studies of isoprenoids biosynthesis and should be applicable to other eukaryotic and prokaryotic (micro)organisms after adaptation of some organism-dependent steps. This methodology also opens the way to ¹³C-metabolic flux analysis of isoprenoid biosynthesis.