Terpene Glycosides from Sanguisorba officinalis and Their Anti-Inflammatory Effects

Abstract

Two new terpene glycosides (<b>1</b>-<b>2</b>) along with two known analogs (<b>3</b>-<b>4</b>) were obtained from the root of <i>Sanguisorba officinalis,</i> which is a common traditional Chinese medicine (TCM). Their structures were elucidated by nuclear magnetic resonance (NMR), electrospray ionization high resolution mass spectrometry (HRESIMS), and a hydrolysis reaction, as well as comparison of these data with the literature data. Compounds <b>1</b>-<b>4</b> exhibited anti-inflammatory properties <i>in vitro</i> by attenuating the production of inflammatory mediators, such as nitric oxide (NO) as well as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). An anti-inflammatory assay based on the zebrafish experimental platform indicated that compound <b>1</b> had good anti-inflammatory activity in vivo by not only regulating the distribution, but also by reducing the amount of the macrophages of the zebrafish exposed to copper sulfate.