Identification of an Anti-MRSA Cyclic Lipodepsipeptide, WBP-29479A1, by Genome Mining of <i>Lysobacter antibioticus</i>

Abstract

<i>Lysobacter</i> are ubiquitous in the environment but remain largely underexplored, although the bacteria are considered "peptide specialists". Here, we identified a new cyclic lipodepsipeptide, WBP-29479A1 (<b>1</b>), through genome mining of <i>L. antibioticus</i> ATCC 29479. <b>1</b> is biosynthesized by a large NRPS gene cluster, and its structure, including the six nonproteinogenic residues and 3-hydroxy fatty acid, was determined by extensive spectroscopic analyses and chemical derivatization. <b>1</b> exhibits potent anti-MRSA activity in a menaquinone-dependent manner.