Use of Extracellular Matrix Hydrogel from Human Placenta to Restore Hair-Inductive Potential of Dermal Papilla Cells

Abstract

<b>Aim:</b> To explore the feasibility of human placenta extracellular matrix (HPECM) hydrogel in restoring the hair-inductive capacity of high-passaged (P8) dermal papilla cells (DPCs) for hair follicle regeneration. <b>Materials & methods:</b> HPECM hydrogel was prepared following decellularization and enzymatic solubilization treatment. DPCs isolated from human scalp were cultured in 2D and 3D environments. The hair-inductive ability of DPCs was assessed by quantitative RT-PCR, immunofluorescence staining, immunoblotting and patch assay. <b>Results:</b> DPCs (P8) formed spheres when cultured on the HPECM hydrogel. The expression levels of Versican, ALP, and <i>β</i>-catenin were restored in the DP spheres. HPECM hydrogel-cultured DP spheres co-grafted with newborn mouse epidermal cells regenerated new hair follicle. <b>Conclusion:</b> HPECM hydrogel successfully restores the hair-inductive capacity of high-passaged DPCs.