Effects of natural wing damage on flight performance in <i>Morpho</i> butterflies: what can it tell us about wing shape evolution?

Abstract

Flying insects frequently experience wing damage during their life. Such irreversible alterations of wing shape affect flight performance and ultimately fitness. Insects have been shown to compensate for wing damage through various behavioural adjustments, but the importance of damage location over the wings has been scarcely studied. Using natural variation in wing damage, here we tested how the loss of different wing parts affect flight performance. We quantified flight performance in two species of large butterflies, Morpho helenor and M. achilles, caught in the wild, and displaying large variation in the extent and location of wing damage. We artificially generated more severe wing damage in our sample to contrast natural vs. higher magnitude of wing loss. Wing shape alteration across our sample was quantified using geometric morphometrics to test the effect of different damage distributions on flight performance. Our results show that impaired flight performance clearly depends on damage location over the wings, pointing out a relative importance of different wing parts for flight. Deteriorated forewings leading edge most crucially affected flight performance, specifically decreasing flight speed and proportion of gliding flight. In contrast, most frequent natural damage such as scattered wing margin had no detectable effect on flight behaviour. Damages located on the hindwings – although having a limited effect on flight – were associated with reduced flight height, suggesting that fore- and hindwings play different roles in butterfly flight. By contrasting harmless and deleterious consequences of various types of wing damage, our study points at different selective regimes acting on morphological variations of butterfly wings.