Thermomonas aquatica sp. nov., isolated from an industrial wastewater treatment plant

Abstract

A white-coloured, Gram-stain-negative, aerobic, rod-shaped bacterium (designated strain SY21^T) was isolated from waste-activated sludge. Optimal growth occurred at 28 °C and pH 7.0. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain SY21^T exhibited 16S rRNA gene sequence similarities of 95.5-98.0 % to <i>Thermomonas</i> species and clustered with the type species of the genus <i>Thermomonas</i>. In strain SY21^T, the predominant respiratory quinone was ubiquinone Q-8, and the cellular fatty acids consisted mainly of iso-C_{15 : 0}, C_{16 : 0}, iso-C_{11 : 0} 3-OH, summed feature 3 and summed feature 9. The major polar lipids were phosphatidylcholine, phosphatidylglycerol and phosphatidylethanolamine. The genomic DNA G+C content was determined to be 67.9 mol% and the DNA-DNA relatedness between strain SY21^T and the closest phylogenetically related strain, <i>Thermomonas carbonis</i> KCTC 42013^T, was 35.0±0.1 %. Based on the distinct phenotypic, chemotaxonomic and phylogenetic properties, strain SY21^T represents a novel species of the genus <i>Thermomonas</i>, for which the name <i>Thermomonas aquatica</i> sp. nov. is proposed. The type strain is SY21^T (=KCTC 62191^T=NBRC 113114^T).