Synergistic Antioxidant Activity of Size Controllable chitosan-templated Prussian Blue Nanoparticle

Abstract

<b>Aim:</b> Prussian blue nanoparticles (PB NPs) have been reported as excellent antioxidant agents owing to their ability to scavenge reactive oxygen species. However, their poor stability <i>in vivo</i> limits their use in biomedical applications. <b>Materials & methods:</b> In this study, we developed chitosan-templated PB NPs using water-soluble chitosan samples with molecular weights ranging from 3 to 100 kDa, which stabilized the PB NPs and improved their antioxidant activity. <b>Results & conclusion:</b> The chitosan-templated PB NPs coordinated with the optimal chitosan molecular weight had uniform sphere-like particles, improved stability and effective scavenging activity of <i>in vitro</i> reactive oxygen species generation in murine fibroblast cells stimulated by oxidative stress agents without any cytotoxicity, implying that they could be promising antioxidant agents.