Genome-Wide Identification of Long Noncoding RNAs and Their Responses to Salt Stress in Two Closely Related Poplars

Abstract

Long noncoding RNAs (lncRNAs) are involved in various biological regulatory processes, but their roles in plants resistance to salt stress remain largely unknown. To systematically explore the characteristics of lncRNAs and their roles in plant salt responses, we conducted strand-specific RNA-sequencing of four tissue types with salt treatments in two closely related poplars (<i>Populus euphratica</i> and <i>Populus alba</i> var. <i>pyramidalis</i>), and a total of 10,646 and 10,531 lncRNAs were identified, respectively. These lncRNAs showed significantly lower values in terms of length, expression, and expression correction than with mRNA. We further found that about 40% and 60% of these identified lncRNAs responded to salt stress with tissue-specific expression patterns across the two poplars. Furthermore, lncRNAs showed weak evolutionary conservation in sequences and exhibited diverse regulatory styles; in particular, tissue- and species-specific responses to salt stress varied greatly in two poplars, for example, 322 lncRNAs were found highly expressed in <i>P. euphratica</i> but not in <i>P. alba</i> var. <i>pyramidalis</i> and 3,425 lncRNAs were identified to be species-specific in <i>P. euphratica</i> in response to salt stress. Moreover, tissue-specific expression of lncRNAs in two poplars were identified with predicted target genes included <i>Aux/IAA</i>, <i>NAC</i>, <i>MYB</i>, involved in regulating plant growth and the plant stress response. Taken together, the systematic analysis of lncRNAs between sister species enhances our understanding of the characteristics of lncRNAs and their roles in plant growth and salt response.