Bioactivity guided isolation and structural characterization of the antidiabetic and antioxidant compound from bark extract of Avicennia officinalis L

Abstract

The present study was aimed at evaluating the antidiabetic activity of the ethanol bark extract of a mangrove plant Avicennia officinalis (AOEB) in streptozotocin (STZ)-induced diabetic mice. The study revealed AOEB could lower the blood glucose level significantly (p < .05) in diabetic mice at dose of 200 mg/kg b.w. The elevated total cholesterol, triglyceride, liver toxicity makers (SGOT and SGPT) and urea level were found to be ameliorated. The in vitro bioactivity-guided assay of AOEB led to isolation of a bioactive compound that inhibits the carbohydrate metabolizing enzymes (α-amylase and α-glucosidase) and also scavenging the DPPH, ABTS and superoxide radicals. The structure of the isolated compound was analyzed by different spectral techniques as FT-IR, CHNS, 1H NMR, 13C NMR, MS. Further, the isolated compound was analyzed by HPLC. However, further studies are required to elucidate the exact structure of the isolated compound.