Zwitterionic Polymer-Grafted Polylactic Acid Vascular Patches Based on a Decellularized Scaffold for Tissue Engineering

Abstract

More than 10 million people suffer from cardiovascular diseases, and diseased blood vessels need to be treated with vascular patches. For a vascular patch, good affinity for endothelial progenitor cells is a key factor in promoting the formation of endothelial tissue—endothelialization. To construct such a vascular patch with good cell affnity, in this work, we first synthesized a reactive zwitterionic organophosphate containing a phosphorylcholine headgroup: 6-(acryloyloxy)hexyl-2-(N-isopropyl-N,N-dimethylammonio)ethyl phosphate (AHEP). We then grafted AHEP onto a polylactic acid (PLA)-coated decellularized scaffold to obtain a vascular patch. Its hydrophilicity and biocompatibility were investigated. Its in vivo performance was also examined in a pig model with B-ultrasonography, Doppler spectra, and computed tomography angiography. The vascular patch demonstrated a nonhemolytic property, noncytotoxicity, long in vitro coagulation times, the strong ability to resist platelet adhesion, and a good affinity for endothelial progenitor cells. The vascular patch was able to maintain the long-term patency (5 months) of surgical arteries. Hence, the zwitterionic polymer-grafted PLA vascular patch may be a promising candidate for vascular tissue engineering.